Diadophis punctatus or the western ringneck snake, is part of the Colubridae family, which is a family of snakes known for their rear fangs. Some of the rear-fanged and non-fanged snakes in this family have a Duvernoy's gland, which is located above the rear fangs. This gland is believed to have toxic secretions. Although a few Colibridae snakes are identified as venomous, some even considered deadly, the genus, Diadophis, is not considered to be venomous. However, there are conflicting reports in the literature showing venom-like reactions to a bite from this type of snake. In order to investigate whether this snake is venomous, our lab is going to collect the venom from the snake, separate the components using HPLC and perform assays to determine the activity and amount of enzymes. To work out the HPLC and assay methods, the Western Diamondback Rattlesnake venom was obtained through Sigma Aldrich. Separation using HPLC was successful using a C18 Reverse phase HPLC column, 0.1%TFA buffer and 80% acetonitrile in 0.1%TFA buffer.