Fungi are known to use many complex modes of nutrition, but due to the difficultly of cultivation, the nutritional habits of certain fungal species can not be observed directly in an experimental setting. In recent decades, the study of isotopes has been a new approach to the determination of fungal trophic strategies. This research attempts to resolve the trophic status of Schenella pityophilus, a hypogeous fungal species native to the Pacific Northwest. Using isotopic ratios of fruiting bodies and organic matter collected from plants and substrate, Schenella pityophilus was compared to a known mycorrhizal species, Rhizopogon truncatus. This research concluded that Schenella pityophilus is likely saprotrophic as is was depleted in δ15N and enriched in δ13C relative to the known mycorrhizal species. The study of isotopes can give us insight into systems of nutrient cycling. Isotopes therefore play an important role in mycological research and the understanding of complex ecosystems.