Isolation of Secondary Metabolites from Mahonia aquifolium

Jesse Korber

Mahonia aquifolium is an evergreen plant native to the Pacific Northwest. Historically, this plant was believed to possess antibiotic properties and was used to treat various skin ailments such as psoriasis and eczema. Previous research into the alkaloid constituents of M. aquifolium revealed the presence of a documented antibiotic, berberine. Other chemical constituents of M. aquifolium have yet to be investigated. In order to gain insight into the terpenoid content (a diverse class of biologically active compounds) stem and root bark tissue of M. aquifolium was analyzed. A room temperature extraction was carried out with 80% ethanol. The concentrated crude extract was partitioned with ethyl acetate followed by chloroform. Initial evaluation with TLC indicates the presence of organics in the chloroform and ethyl acetate extracts. Extracts will be partitioned using column chromatography and their chemical constituents evaluated using GC-MS.