Mycorrhizal Database

 
SOU
Morphology

Anatomy
OM: outer mantle
IM: inner mantle

DNA

(ITS1-F/ITS4)†

4

Unbranched

Mantle white, reflective, squishy, cottony

Emanting hyphae aseptate

OM: net prosenchyma

PCR:

HinFI: 411/286/215/130

TaqI: 364/300/184

AluI:

20

Monopodial, unbranched

Mantle tan to white, finely grainy, matte, very squishy, not well differentiated

OM: net prosenchyma

PCR:

HinFI:

TaqI:

AluI:

31

Irregular to unbranched

Mantle white, smooth, matte to shiny

OM/IM: regular synenchyma 7 x 7 µm

PCR: 758

HinFI: 344/225/145

TaqI: 348/338/68

AluI: 381/169/73

35

Irregular

Mantle white iridescent with patches of tan, felty, reflective

Emanating septate hyphae 4 µm thick, bent and branching

OM: felt prosenchyma 4 µm thick

IM: net prosenchyma

PCR: 794

HinFI: 384/284/88

TaqI: 113*/81/76

AluI:

39

Unbranched to irregular

Mantle cream tan, reflective white patches, smooth to finely grainy

Emanating aseptate hyphae 3 µm thick

OM: interlocking irregular synenchyma 3 x 6 µm

PCR:

HinFI:

TaqI:

AluI:

13

Irregularly branched to unbranched, bent

Mantle brown, to dark brown often with tan apices, smooth to finely grainy, thin

OM: regular synenchyma

IM: non-interlocking irregular synenchyma

PCR:

HinFI:

TaqI:

AluI:

2

Straight, monopodial to unbranched

Mantle brown with cream apices, smooth and shiny

OM/IM: regular synenchyma 6 x 10 µm

PCR:

HinFI:

TaqI:

AluI:

23

Unbranched or irregularly branched

Mantle brown, finely grainy, matte to shiny medium thickness, some with white apices

Cystidia, awn-shaped (aciculate) 50 x 3 µm, clear brown

OM/IM: regular synenchyma

PCR: 581

HinFI: 266/225/103

TaqI: 266/200/62

AluI: 460/85

30

Coralloid, irregularly branched to unbranched, bent to tortuous

Mantle brown-orange (yellow), younger ones cream, matte, smooth, some apices light colored

Tuber sp.

OM: interlocking irregular to non-interlocking irregular synenchyma 5 x 7 µm

IM: non-interlocking irregular synenchyma to interlocking irregular synenchyma

PCR: 766

HinFI: 361/340/127

TaqI: 425**/183/87

AluI: 311/179/150

38

Irregular branching

Mantle brown (red tone), finely grainy, shiny and reflective, very brittle

Boletus zelleri

OM: between net and interlocking irregular syenchyma 4 x 10 µm

IM: net synenchyma

PCR: 804

HinFI:18/290/144/103

TaqI: 132*/111/86/79/72

AluI: 81/158

9

 

unbranched to irregularly branched, straight

Mantle tan to light yellow, matte and grainy

 

Emanating, septate hyphae

OM: non-interlocking irregular synenchyma

PCR:

HinFI:

TaqI:

AluI:

1

Monopodial pinnate to unbranched, straight

Mantle orange, smooth to finely grainy, matte; sometimes with black aseptate hyphae encroaching

OM/IM: non-interlocking irregular synenchyma 5 x 8 µm

PCR: 854

HinFI: 320*280

TaqI: 430*/182/86

AluI:

26

Unbranched to irregularly branched and bent to club shaped

Mantle yellow-orange, finely grainy, matte to shiny

Emanating, verrucose, curved, septate hyphae with rare clamp connections. Restricted point mycelial strands sometimes present

OM: net synenchyma 3 x 7 µm

IM: net prosenchyma to net synenchyma

PCR:

HinFI:

TaqI:

AluI:

10

Irregularly branched and bent

Mantle gray and white, smooth to felty, matte to slightly shiny

OM: net prosenchyma

PCR: 667

HinFI: 322/233/153

TaqI: 307/251/146

AluI:

32

Irregularly branched, wrapped around dead root

Mantle white, grey outer sheath, tan with darker Hartig net visible, felty , and matte

Emanating rare, aseptate

OM: net prosenchyma in parallel pattern like finger print, 4 x 10 µm

IM: net synenchyma 2 x 5 µm

PCR: 792

HinFI: 406/252/178

TaqI: 362**/292/266/76/70

AluI:

14
Unbranched, straight Mantle gray iridescent, wooly, reflective

Emanating, septate hyphae, 4 µm wide with papillae, and rare H-shaped anastomoses. Loose-differentiated mycelial strands present with septa; clamp connections

OM: felt prosenchyma

PCR: 712

HinFI: 349/179d

TaqI: 359/235/84

AluI:

8

Unbranched and bent

Mantle gray, white, shiny

Mycelial strands extensive loose-differentiated; emanating, septate, hyphae; clamp connections

OM: felt prosenchyma to net prosynchyma

PCR: 795

HinFI: 345/208/193

TaqI: 347/169/101/71/62

AluI:

22

Tips unbranched or irregular to coralloid.

Mantle brown with dense cottony hyphae, many tips with cream apices, some older tips pure brown. Most tips shiny but a few reflective.

Dense cottony emanating hyphae clear, thin, curved, 5 µm wide with septa; clamps. Mycelial strands developing from dense hyphae; clamp connections

OM: felt prosenchyma and net synenchyma

IM: net synenchyma 5 x 10 µm to non-interlocking irregular synenchyma

PCR: 619

HinFI: 365/349

TaqI: 286/224/82/66

AluI:

18

Unbranched, bent

Mantle brown (red), shiny, grainy,

Emanating septate hyphae; clamp connections

OM: net prosenchyma

PCR: 798

HinFI: 415/305

TaqI: 309/282/165/133

AluI: 451/143/78

25

Unbranched, straight

Mantle brown, shiny

Emanating septate hyphae, 5 µm wide with clamp connections, curved ; clamp connections

OM: non-interlocking irregular synenchyma 5 x 10 µm

PCR:

HinFI:

TaqI:

AluI:

27

Unbranched to irregularly branched, bent

Mantle brown, reflective, coarsely grainy, cottony

Abundant, emanating, cottony, forked, septate hyphae 4.5 µm wide

OM/IM: regular synenchyma cells 7 x 10 µm with thick walls regular synenchyma

PCR: 1000/782

HinFI: 565/409/303

TaqI: 315/293/171/142/82

AluI:

34

Irregular branching

Mantle brown some with cream apices; smooth, shiny; mantle pattern visible in dissecting scope

Emanating hyphae branched, septate, 5-7 µm wide, clear, verrucose areas

OM: regular synenchyma with some non-interlocking irregular synenchyma shapes, thick hyphal walls

IM: regular synenchyma 10 x 10 µm thick walls

PCR: 658

HinFI: 341/328

TaqI: 390/260

AluI:

16

Unbranched and straight

Mantle brown, (red), finely grainy

Abundant aseptate hyphae and restricted point, differentiated, cream mycelial strands

OM: net prosenchyma cells 2.5 x 6 µm

IM: net synenchyma 3 x 4 µm.

PCR:

HinFI: 384/279

TaqI: 290/261/150

AluI:

17

Unbranched, club-shaped

Mantle orange-brown, smooth, shiny

Loose, undifferentiated restricted point mycelial strands present

OM: net prosenchyma

IM: interlocking synenchyma emanating aseptate hyphae 7 µm, with red oil-like bodies.

PCR: 770

HinFI: 418/307

TaqI: 314*/287/168/137

AluI:

29

Unbranched

Mantle brown, cottony, club-shaped, 1 mm

Emanating, aseptate hyphae with abundant forks

OM: regular synenchyma thick walled

IM: regular synenchyma

PCR:

HinFI:

TaqI:

AluI:

36

Unbranched and irregular branched

Mantle brown, wooly, shiny, thick

Aseptate emanating hyphae with few branches

OM: felt prosenchyma 2 µm wide

IM: net prosenchyma (vague star patterns)

PCR: 797

HinFI: 436/305

TaqI: 302*

AluI:

3

Unbranched to branched, bent to club-shaped

Mantle black, grainy, matte, thick

Cenococcum geophilum

Emanating hyphae black, 5 µm thick, abundant, septa rare

OM/IM: net synenchyma with star-like pattern (radiating thick walled isodiametric cells 10-20 µm)

PCR: 578

HinFI: 153/112/94

TaqI: 248/138/68

AluI: 377/188

6

Bent, irregularly branched.

Mantle black, matte; smooth to finely grainy

Mycelial strands slightly differentiated sometimes present, aseptate, 10-50 µm wide.

OM: irregular synenchyma with star-like pattern; cells 3 x 13 µm.

PCR: 735

HinFI: 182/165d/130

TaqI: 345/226/84

AluI:

21

Irregular and bent.

Mantle black, coarse grainy, matte, medium thickness

Tuber rufum

Emanating hyphae common, curved, brown clear, 4 µm wide, thick walled, branching, aseptate.

OM: interlocking irregular synenchyma with thick hyphal walls

PCR: 577

HinFI: 254/213/97

TaqI: 266/200/64

AluI: 460/85

5

Unbranched to irregularly branched.

Mantle black (dark brown) warty, shiny

Emanating hyphae, clear brown 3.5 µm wide, common, septate; clamp connections

OM: non-interlocking irregular synenchyma

PCR:

HinFI:

TaqI:

AluI:

24
Bristle-like (aciculate) cystidia 3 x 30 um non-branched to branched to monopodial pinnate, tips bent to straight

OM: interlocking irregular synenchyma 6 x 10 µm

IM: non-interlocking synenchyma 5 x 15 µm

PCR:

HinFI:

TaqI:

AluI:

7
Fusiform cystidia,staight, bent, unbranched and branched
Net prosenchymatan, grainy, amorphous, matte

PCR:

HinFI: 320/215/138

TaqI: 336d/226/84

AluI:

11
Irregularly branched to unbranched, brown (red), finely grainy, shiny

OM: regular synenchyma cells 8 x 10 um

IM: interlocking irregular synenchyma, cystidia 5 x 275 µm

PCR: 860

HinFI: 400/170d/108

TaqI: 443*/275

AluI:

40
Young are white older are brown, yellow apices, and matte with cystidia, abundant aciculate cystidia, pyramidal to irregular branching

OM: non-interlocking irregular synenchyma 7x10 µm

IM: non-interlocking irregular synenchyma

PCR: 566

HinFI: 268/226/103

TaqI: 269/204

AluI: 464/86

41
White, wooly, thick, 2mm long .3-.5 wide, big tip, abundant cystidia

OM: net prosenchyma

IM: net prosenchyma

PCR:

HinFI:

TaqI:

AluI:

37
Mantle red with abundant cystidia with crytalline structures on surface

OM: cystidia covered coating

IM: net prosenchyma (Hartig net difficult to see so may not be ectomyorrhizal?)

PCR: 787

HinFI: 415/262

TaqI: 374/339

AluI:

* : Sum of restriction products significantly less than primary product. ** : Sum of restriction products significantly more than primary product. d:doublet.

†: Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2:113-118.

 

 

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