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SOU
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Morphology
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Anatomy |
DNA (ITS1-F/ITS4) |
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4
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Unbranched Mantle white, reflective, squishy, cottony |
Emanting hyphae aseptate OM: net prosenchyma |
PCR: HinFI: 411/286/215/130 TaqI: 364/300/184 AluI: |
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20
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Monopodial, unbranched Mantle tan to white, finely grainy, matte, very squishy, not well differentiated |
OM: net prosenchyma
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PCR: HinFI: TaqI: AluI: |
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31
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Irregular to unbranched Mantle white, smooth, matte to shiny |
OM/IM: regular synenchyma 7 x 7 µm
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PCR: 758 HinFI: 344/225/145 TaqI: 348/338/68 AluI: 381/169/73 |
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35
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Irregular Mantle white iridescent with patches of tan, felty, reflective |
Emanating septate hyphae 4 µm thick, bent and branching OM: felt prosenchyma 4 µm thick IM: net prosenchyma |
PCR: 794 HinFI: 384/284/88 TaqI: 113*/81/76 AluI: |
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39
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Unbranched to irregular Mantle cream tan, reflective white patches, smooth to finely grainy |
Emanating aseptate hyphae 3 µm thick OM: interlocking irregular synenchyma 3 x 6 µm |
PCR: HinFI: TaqI: AluI: |
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13
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Irregularly branched to unbranched, bent Mantle brown, to dark brown often with tan apices, smooth to finely grainy, thin |
OM: regular synenchyma IM: non-interlocking irregular synenchyma |
PCR: HinFI: TaqI: AluI: |
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2
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Straight, monopodial to unbranched Mantle brown with cream apices, smooth and shiny |
OM/IM: regular synenchyma 6 x 10 µm
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PCR: HinFI: TaqI: AluI: |
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23
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Unbranched or irregularly branched Mantle brown, finely grainy, matte to shiny medium thickness, some with white apices |
Cystidia, awn-shaped (aciculate) 50 x 3 µm, clear brown OM/IM: regular synenchyma |
PCR: 581 HinFI: 266/225/103 TaqI: 266/200/62 AluI: 460/85 |
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30
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Coralloid, irregularly branched to unbranched, bent to tortuous Mantle brown-orange (yellow), younger ones cream, matte, smooth, some apices light colored Tuber sp. |
OM: interlocking irregular to non-interlocking irregular synenchyma 5 x 7 µm IM: non-interlocking irregular synenchyma to interlocking irregular synenchyma |
PCR: 766 HinFI: 361/340/127 TaqI: 425**/183/87 AluI: 311/179/150 |
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38
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Irregular branching Mantle brown (red tone), finely grainy, shiny and reflective, very brittle Boletus zelleri |
OM: between net and interlocking irregular syenchyma 4 x 10 µm IM: net synenchyma |
PCR: 804 HinFI:18/290/144/103 TaqI: 132*/111/86/79/72 AluI: 81/158 |
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9
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unbranched to irregularly branched, straight Mantle tan to light yellow, matte and grainy |
Emanating, septate hyphae OM: non-interlocking irregular synenchyma |
PCR: HinFI: TaqI: AluI: |
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1
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Monopodial pinnate to unbranched, straight Mantle orange, smooth to finely grainy, matte; sometimes with black aseptate hyphae encroaching |
OM/IM: non-interlocking irregular synenchyma 5 x 8 µm
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PCR: 854 HinFI: 320*280 TaqI: 430*/182/86 AluI: |
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26
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Unbranched to irregularly branched and bent to club shaped Mantle yellow-orange, finely grainy, matte to shiny |
Emanating, verrucose, curved, septate hyphae with rare clamp connections. Restricted point mycelial strands sometimes present OM: net synenchyma 3 x 7 µm IM: net prosenchyma to net synenchyma |
PCR: HinFI: TaqI: AluI: |
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10
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Irregularly branched and bent Mantle gray and white, smooth to felty, matte to slightly shiny |
OM: net prosenchyma
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PCR: 667 HinFI: 322/233/153 TaqI: 307/251/146 AluI: |
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32
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Irregularly branched, wrapped around dead root Mantle white, grey outer sheath, tan with darker Hartig net visible, felty , and matte |
Emanating rare, aseptate OM: net prosenchyma in parallel pattern like finger print, 4 x 10 µm IM: net synenchyma 2 x 5 µm |
PCR: 792 HinFI: 406/252/178 TaqI: 362**/292/266/76/70 AluI: |
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14
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Unbranched, straight Mantle gray iridescent, wooly, reflective
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Emanating, septate hyphae, 4 µm wide with papillae, and rare H-shaped anastomoses. Loose-differentiated mycelial strands present with septa; clamp connections OM: felt prosenchyma |
PCR: 712 HinFI: 349/179d TaqI: 359/235/84 AluI: |
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8
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Unbranched and bent Mantle gray, white, shiny |
Mycelial strands extensive loose-differentiated; emanating, septate, hyphae; clamp connections OM: felt prosenchyma to net prosynchyma |
PCR: 795 HinFI: 345/208/193 TaqI: 347/169/101/71/62 AluI: |
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22
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Tips unbranched or irregular to coralloid. Mantle brown with dense cottony hyphae, many tips with cream apices, some older tips pure brown. Most tips shiny but a few reflective. |
Dense cottony emanating hyphae clear, thin, curved, 5 µm wide with septa; clamps. Mycelial strands developing from dense hyphae; clamp connections OM: felt prosenchyma and net synenchyma IM: net synenchyma 5 x 10 µm to non-interlocking irregular synenchyma |
PCR: 619 HinFI: 365/349 TaqI: 286/224/82/66 AluI: |
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18
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Unbranched, bent Mantle brown (red), shiny, grainy, |
Emanating septate hyphae; clamp connections OM: net prosenchyma |
PCR: 798 HinFI: 415/305 TaqI: 309/282/165/133 AluI: 451/143/78 |
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25
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Unbranched, straight Mantle brown, shiny |
Emanating septate hyphae, 5 µm wide with clamp connections, curved ; clamp connections OM: non-interlocking irregular synenchyma 5 x 10 µm |
PCR: HinFI: TaqI: AluI: |
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27
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Unbranched to irregularly branched, bent Mantle brown, reflective, coarsely grainy, cottony |
Abundant, emanating, cottony, forked, septate hyphae 4.5 µm wide OM/IM: regular synenchyma cells 7 x 10 µm with thick walls regular synenchyma |
PCR: 1000/782 HinFI: 565/409/303 TaqI: 315/293/171/142/82 AluI: |
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34
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Irregular branching Mantle brown some with cream apices; smooth, shiny; mantle pattern visible in dissecting scope |
Emanating hyphae branched, septate, 5-7 µm wide, clear, verrucose areas OM: regular synenchyma with some non-interlocking irregular synenchyma shapes, thick hyphal walls IM: regular synenchyma 10 x 10 µm thick walls |
PCR: 658 HinFI: 341/328 TaqI: 390/260 AluI: |
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16
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Unbranched and straight Mantle brown, (red), finely grainy |
Abundant aseptate hyphae and restricted point, differentiated, cream mycelial strands OM: net prosenchyma cells 2.5 x 6 µm IM: net synenchyma 3 x 4 µm. |
PCR: HinFI: 384/279 TaqI: 290/261/150 AluI: |
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17
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Unbranched, club-shaped Mantle orange-brown, smooth, shiny |
Loose, undifferentiated restricted point mycelial strands present OM: net prosenchyma IM: interlocking synenchyma emanating aseptate hyphae 7 µm, with red oil-like bodies. |
PCR: 770 HinFI: 418/307 TaqI: 314*/287/168/137 AluI: |
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29
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Unbranched Mantle brown, cottony, club-shaped, 1 mm |
Emanating, aseptate hyphae with abundant forks OM: regular synenchyma thick walled IM: regular synenchyma |
PCR: HinFI: TaqI: AluI: |
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36
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Unbranched and irregular branched Mantle brown, wooly, shiny, thick |
Aseptate emanating hyphae with few branches OM: felt prosenchyma 2 µm wide IM: net prosenchyma (vague star patterns) |
PCR: 797 HinFI: 436/305 TaqI: 302* AluI: |
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3
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Unbranched to branched, bent to club-shaped Mantle black, grainy, matte, thick Cenococcum geophilum |
Emanating hyphae black, 5 µm thick, abundant, septa rare OM/IM: net synenchyma with star-like pattern (radiating thick walled isodiametric cells 10-20 µm) |
PCR: 578 HinFI: 153/112/94 TaqI: 248/138/68 AluI: 377/188 |
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6
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Bent, irregularly branched. Mantle black, matte; smooth to finely grainy |
Mycelial strands slightly differentiated sometimes present, aseptate, 10-50 µm wide. OM: irregular synenchyma with star-like pattern; cells 3 x 13 µm. |
PCR: 735 HinFI: 182/165d/130 TaqI: 345/226/84 AluI: |
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21
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Irregular and bent. Mantle black, coarse grainy, matte, medium thickness Tuber rufum |
Emanating hyphae common, curved, brown clear, 4 µm wide, thick walled, branching, aseptate. OM: interlocking irregular synenchyma with thick hyphal walls |
PCR: 577 HinFI: 254/213/97 TaqI: 266/200/64 AluI: 460/85 |
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5
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Unbranched to irregularly branched. Mantle black (dark brown) warty, shiny |
Emanating hyphae, clear brown 3.5 µm wide, common, septate; clamp connections OM: non-interlocking irregular synenchyma |
PCR: HinFI: TaqI: AluI: |
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24
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Bristle-like (aciculate) cystidia 3 x 30 um non-branched
to branched to monopodial pinnate, tips bent to straight
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OM: interlocking irregular synenchyma 6 x 10 µm IM: non-interlocking synenchyma 5 x 15 µm |
PCR: HinFI: TaqI: AluI: |
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7
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Fusiform cystidia,staight, bent, unbranched and branched
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Net prosenchymatan, grainy, amorphous, matte
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PCR: HinFI: 320/215/138 TaqI: 336d/226/84 AluI: |
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11
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Irregularly branched to unbranched, brown (red), finely
grainy, shiny
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OM: regular synenchyma cells 8 x 10 um IM: interlocking irregular synenchyma, cystidia 5 x 275 µm |
PCR: 860 HinFI: 400/170d/108 TaqI: 443*/275 AluI: |
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40
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Young are white older are brown, yellow apices, and matte
with cystidia, abundant aciculate cystidia, pyramidal to irregular branching
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OM: non-interlocking irregular synenchyma 7x10 µm IM: non-interlocking irregular synenchyma |
PCR: 566 HinFI: 268/226/103 TaqI: 269/204 AluI: 464/86 |
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41
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White, wooly, thick, 2mm long .3-.5 wide, big tip, abundant
cystidia
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OM: net prosenchyma IM: net prosenchyma |
PCR: HinFI: TaqI: AluI: |
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37
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Mantle red with abundant cystidia with crytalline structures
on surface
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OM: cystidia covered coating IM: net prosenchyma (Hartig net difficult to see so may not be ectomyorrhizal?) |
PCR: 787 HinFI: 415/262 TaqI: 374/339 AluI: |
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* : Sum of restriction products significantly less than primary product. ** : Sum of restriction products significantly more than primary product. d:doublet. : Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2:113-118. |
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